Real time quantitative PCR technology, as a kind of molecular biological technique, polymerase chain reaction (Polymerase chain reaction, PCR) is widely used in medicine and biology laboratory, in the detection of small fragments of DNA, PCR has been proved to be a very effective technique, the target area can be quickly amplified DNA. For example, to determine whether the sample will be shown in the performance of a genetic disease map, the diagnosis of infectious diseases, identification of the authenticity of biological origin ingredients.

Real time quantitative PCR (Real-time Quantitative PCR Detecting System, referred to as QPCR), is a recently developed new PCR technology in qPCR system, fluorescent probe can be combined effectively in PCR primer to increase the specificity of the reaction. Compared with the common PCR technology, the sensitivity, speed, accuracy and automation level of qPCR were significantly improved, the biggest advantage is quantitative, which has been widely used in quantitative gene expression and quantitative detection of pathogenic bacteria